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Characterization of novel clonal murine endothelial cell lines with an extended life span

  • Cell and Tissue Models
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Summary

A murine endothelial cell line was recently established from microvessels that had invaded a subcutaneous sponge implant (Dong, Q. G.; Bernasconi, S.; Lostaglio, S., et al. Arterioscl. Thromb. Vasc. Biol. 17:1599–1604; 1997). From these sponge-induced endothelial (SIE) cells, we have isolated two subpopulations endowed with different phenotypic properties. Clone SIE-F consists of large, highly spread cells that have a relatively slow growth rate, form contact-inhibited monolayers, do not grow under anchorage-independent conditions, express elevated levels of thrombospondin-1 (TSP-1) and are not tumorigenic in vivo. In contrast, clone SIE-S2 consists of small, spindle-shaped cells that have a high proliferation rate, do not show contact-inhibition, grow under anchorage-independent conditions, express very low levels of TSP-1 and are tumorigenic in vivo. Both clones express the endothelial markers vascular endothelial-cadherin and vascular intercellular adhesion molecule-1, but do not express CD31 and E-selectin. In addition, SIE-S2 cells, but not SIE-F cells, express the α-smooth muscle actin isoform. SIE-S2 cells, but not SIE-F cells, are able to form branching tubes in fibrin gels. The SIE-F and SIE-S2 clones, which have properties of nontransformed and transformed cells, respectively, should provide useful tools to investigate physiological and pathological processes involving vascular endothelium.

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Authors and Affiliations

  1. Dibit, Scientific Institute San Raffaele, I-20132, Milan, Italy

    Ugo Cavallaro, Vera Castelli, Alessandra Perilli & Marco R. Soria

  2. Institute of General Pathology, University of Milan, Italy

    Ugo Cavallaro

  3. Mario Negri Institute for Pharmacological Research, I-24125, Bergamo, Italy

    Romina Dossi & Raffaella Giavazzi

  4. Department of Morphology, University of Geneva Medical Center, CH-1211, Geneva, Switzerland

    Michael S. Pepper & Roberto Montesano

Authors
  1. Ugo Cavallaro
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  2. Vera Castelli
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  3. Alessandra Perilli
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  4. Romina Dossi
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  5. Raffaella Giavazzi
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  6. Michael S. Pepper
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  7. Marco R. Soria
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  8. Roberto Montesano
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Correspondence to Roberto Montesano.

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Cavallaro, U., Castelli, V., Perilli, A. et al. Characterization of novel clonal murine endothelial cell lines with an extended life span. In Vitro Cell.Dev.Biol.-Animal 36, 299–308 (2000). https://doi.org/10.1290/1071-2690(2000)036<0299:CONCME>2.0.CO;2

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  • DOI: https://doi.org/10.1290/1071-2690(2000)036<0299:CONCME>2.0.CO;2

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